Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia Using Polymerase Chain Reaction and a Restriction Endonuclease
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چکیده
Anopheles (Nyssorhynchus) marajoara Galvão & Damasceno (Diptera: Culicidae) is a memberof theAlbitarsis cryptic species complex and is aprimaryvectorofhumanPlasmodium inparts of Brazil. We report the Þrst record of An.marajoara in the department of Cochabamba and conÞrm its presence in the department of Santa Cruz, Bolivia. We also describe a PCR protocol producing a single amplicon ( 340 bp) of a section of the internal transcribed spacer 2 speciÞc for members of the Albitarsis species complex; the polymerase chain reaction protocol did not amplify the DNA of 17 other species in the subgenus Nyssorhynchus. Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments speciÞc to An. marajoara in Bolivia.
منابع مشابه
Cryptic Species in the Anopheles (Nyssorhynchus) albitarsis (Diptera: Culicidae) Complex: Incongruence Between Random Amplified Polymorphic DNA-Polymerase Chain Reaction Identification and Analysis of Mitochondrial DNA COI Gene Sequences.
Random amplified polymorphic DNA (RAPD) diagnostic bands are one tool used to differentiate cryptic mosquito species in the Anopheles albitarsis Complex. Monophyly of four species (A. albitarsis Lynch-Arribálzaga, A. albitarsis B, A. deaneorum Rosa-Freitas, and A. marajoara Galvão & Damasceno) currently identified with the RAPD technique was assessed using sequences of the cytochrome oxidase I ...
متن کاملIdentification of Anopheles (Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes.
Anopheles (Nyssorhynchus) marajoara is a proven primary vector of malaria parasites in Northeast Brazil, and An. deaneorum is a suspected vector in Western Brazil. Both are members of the morphologically similar Albitarsis Complex, which also includes An. albitarsis and an undescribed species, An. albitarsis "B". These four species were recognized and can be identified using random amplified po...
متن کاملIdentification of species related to Anopheles (Nyssorhynchus) albitarsis by random amplified polymorphic DNA-polymerase chain reaction (Diptera: Culicidae).
Species-specific Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) markers were used to identify four species related to Anopheles (Nyssorhynchus) albitarsis Lynch-Arribàlzaga from 12 sites in Brazil and 4 in Venezuela. In a previous study (Wilkerson et al. 1995), which included sites in Paraguay and Argentina, these four species were designated "A", "B", "C" and "D". It was...
متن کاملMolecular Phylogeny of Neotropical Anopheles (Nyssorhynchus) Albitarsis Species Complex (Diptera: Culicidae).
A phylogeny was reconstructed for four species belonging to the Neotropical Anopheles (Nyssorhynchus) albitarsis complex using partial sequences from the mitochondrial cytochrome oxidase I (COI) and NADH dehydrogenase 4 (ND4) genes and the ribosomal DNA ITS2 and D2 expansion region of the 28S subunit. The basis for initial characterization of each member of the complex was by correlated random ...
متن کاملNotes on the holotype of Anopheles marajoara Galvão & Damasceno (Diptera, Culicidae)
Anopheles subgenus Nyssorhynchus is a monophyletic Neotropical group (Sallum et al. 2000, 2002) that currently contains 32 named species (Harbach 2004). The most important vectors of human malaria parasites in Central and South America are members of the subgenus and include An. darlingi Root, 1926, An. albimanus Wiedemann, 1820 and An. marajoara Galvão & Damasceno, 1942 (Conn et al. 2002). Ano...
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